Determination of Ozone Gas Effectiveness as Antifungal Agent

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Determination of Ozone Gas Effectiveness as Antifungal Agent

Objective: Molds and yeasts which are predominant microorganisms in the air of food facility, contaminate foods through airborne and cause microbial spoilage. In addition to their adverse effects on food quality, molds produce mycotoxins which have toxic effects on humans and animals. Nowadays, ozone (O3) applications appear as a green technology used mold prevention and detoxification method in food industry. In this study, the antifungal activity of O3 gas was determined in a controlled air environment.

Material and methods: 0,5 McFarland yeast (Candida parapsilosis, Schizosaccharomyces pombe, Saccharomyces cerevisiae) and mold spore (Aspergillus flavus, Aspergillus parasiticus, Penicillium digitatum, Penicillium expansum, Penicillium roqueforti) suspensions were sprayed into 250 L airtight test cabin and exposed to ozone gas (10.000 mg/hour) for 3, 5, 10 min. Antifungal activity was determined by comparing the number of microorganisms by active- passive sampling before and after ozone application.

Results and conclusion: 100% antifungal effect was detected in all tested microorganisms with 3 minutes of ozone application.

 

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